Synthesis of Chitosan Nanoascorbate Bombyx Mori

Abstract

In the present work, chitosan nanoascorbate Bombyx mori (CSNA) based on high-molecular-weight chitosan (CS) and ascorbic acid (AA) was synthesized by ionotropic gelation in the presence of tripolyphosphate (TPP). The results of the conducted kinetic studies show that in the studied interaction, the order of the reaction of ascorbic acid exceeds the order of the reaction of chitosan. In this study, theoretical studies based on density functional theory (DFT) have performed to comprehend the interaction between of chitosan dimer with AA and sodium tripolyphosphate (TPP) by during the formation of chitosan ascorbate (CSA) nanostructure. Based on existing results, the CS monomer formed of complexes occurs due to the donor-acceptor interaction, which is energetically favorable in all considered inter-actions according to the calculations. The order of values interaction energy de-creases: CS-AА (-68.76 kcal/mol), CS-TPP (-64.58 kcal/mol), which indicates that in the process of formation of CSNA, in most cases, the formation of a donor-acceptor bond occurs between the amino groups of CS with the enol group of AA. The introduction of the aqueous phase led to a drop in the interaction energy. CS nanoparticles can protect ascorbic acid from degradation and improve the stability of AA.