Influence of Herbal Preparations on the Process of Lipid Peroxidation and Enzyme Activity of Rat Liver Mitochondria with Drug Damage

Abstract

The influence of various doses (25 and 50 mg/kg) of the flavonoid quercetin, the monoammonium salt of glycyrrhizic acid (MASGA) as well as the supramolecular complex derived from them (MASGA/quercetin) on the processes of lipid peroxidation (LPO), respiration, and oxidative phosphorylation of mitochondria as well as the activity of mitochondrial respiratory chain enzymes in experimental paracetamol hepatitis were studied. In the work, methods of differential centrifugation, methods for determining the content of the LPO product - malondialdehyde (MDA) using thiobarbituric acid, polarographic methods for determining the functional state and activities of mitochondrial respiratory chain enzymes were used. It has been established that toxic hepatitis develops in experimental rats under the action of paracetamol, characterized by a more than twofold increase in MDA in mitochondria in ascorbate-dependent and NADH (nicotinamide adenine dinucleotide (NAD) + hydrogen (H))-dependent systems. Magnification of the LPO process leads to the inhibition of the respiratory and phosphorylating functions of mitochondria by 35 and 30% compared with intact animals and is accompanied by inhibition of the oxidation of NAD- and FAD-dependent substrates and inhibition of respiratory chain enzymes by approximately 40%. Correction of the identified disorders using various doses of quercetin, MASGA, and the MASGA/quercetin complex showed that all the studied compounds have the ability to restore the detected disorders in mitochondria, but the supramolecular complex MASGA/quercetin at a dose of 25 mg/kg has the greatest stimulating effect on the functional state of mitochondria. It is assumed that the supramolecular complex MASGA/quercetin can serve as the basis for the creation of hepatoprotectors for the treatment of drug-induced hepatitis. For the first time, the influence of the supramolecular complex MASGA/quercetin on the LPO process and the functional state of rat liver mitochondria in experimental toxic hepatitis caused by the administration of paracetamol was studied.