Biochemical Metabolite Analysis: GC Nitrogen and Carbon for Antibacterial Activity and Molecular Identification of Soil-Producing Antibiotic Bacteria

Abstract

In order to combat infections and cure illnesses, fungi and bacteria produce antibiotics. The need for new antibiotic discoveries is a result of antibiotic resistance. Since pathogenic bacteria are common in soil, many techniques have been employed to create strong natural antibiotics against them. Bacteria that produce antibiotics were isolated from soil in this investigation. Taken from the Bhopal region. Of the five isolates, two were able to inhibit Klebsiella pneumoniae and Escherichia coli growth. Although the strains differed in culture, morphological and biochemical tests recognized them as Pseudomonas. Pseudomonas fluorescence and aeruginosa were determined to be the amplified 16S rRNA PCR products after sequencing revealed 98% and 97% similarity scores, respectively. Based on this research, it is possible that Pseudomonas species generate antibiotics that suppress a variety of microorganisms and boost their growth. Regarding their ability to inhibit bacteria, the isolates favored distinct sources of carbon and nitrogen. The culture filtrates that were used were sterile, filter-sterilized, and proteinase K-treated; the sterilized filtrates exhibited greater antibacterial activity. 18.5 And 15.5 mm inhibition zones were shown for streptomycin and culture filtrates. All isolates' antibacterial activity against human pathogens is reported for the first time in this study. Culture filtrate antibacterial activity was significantly decreased by proteinase K. It appears from these results that these three bacteria may be isolates with antibacterial properties. More than 5 mg/mL ciprofloxacin, all bacterial extracts inhibited E. coli growth. As per the study, these species possess the ability to generate antimicrobial compounds that combat microbial infections and strains that are resistant to drugs. Propanoic acid, oxalic acid, phenol, and hexadecanoic acid were among the antimicrobial substances identified by GC-MS in bacterial extracts.