The Acceptable Concentration of Cytokinin and Auxin in The Regeneration of Grape Varieties “In Vitro” In The Content of Ms and DKW Nutritional Condition

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Djonibekova Nafosat Ergash Kizi

Abstract

In this article , the difference in the growth indicators of grape varieties “Toyfi rozovi”, “Rizamat”, “Pobeda (Mers)”, “Husayne beli” in “in vitro” conditions in Murashige and Skoog and Driver and Kuniyuki nutritional condition, the effect of cytokinin and auxin on them and the optimal concentration were determined. In the study, the concentration of auxin NAA (naphthylacetic acid) was 0.01 and 0.5 mg/l, cytokinin BAP (6-benzylaminopurine ) was used 0.1; 0.5; 1.0; 2.0 mg/l. Explants branching length, number of branches, number of leaves, root length, growth indicators were determined. According to the results of Murashige and Skoog nutrient condition, the branching number of explants is high, NAA (naphthylacetic acid) at two concentrations of 0.01 and 0.5 mg/l, BAP (6- benzylaminopurine) concentration of 0.5; 1.0 mg/l was recommended. In Driver and Kuniyuki nutrient condition, the growth and rooting of explants are high, NAA (naphthylacetic acid) in concentration of 0.01 and 0.5 mg/l, BAP (6- benzylaminopurine ) 0.1; 0.5; 1.0 mg/l was recommended.

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